Journal
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
Volume 55, Issue 5, Pages 1662-1665Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.201507097
Keywords
aspergillus; biosynthesis; genetic dereplication; natural products; NRPS
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Funding
- H. L. Snyder Medical Foundation
- Irving S. Johnson Fund of the University of Kansas Endowment
- National Institute of General Medical Sciences [P01-GM084077]
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To reduce the secondary metabolite background in Aspergillus nidulans and minimize the rediscovery of compounds and pathway intermediates, we created a genetic dereplication strain in which we deleted eight of the most highly expressed secondary metabolite gene clusters (more than 244,000 base pairs deleted in total). This strain allowed us to discover a novel compound that we designate aspercryptin and to propose a biosynthetic pathway for the compound. Interestingly, aspercryptin is formed from compounds produced by two separate gene clusters, one of which makes the well-known product cichorine. This raises the exciting possibility that fungi use differential regulation of expression of secondary metabolite gene clusters to increase the diversity of metabolites they produce.
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