Journal
CHINESE CHEMICAL LETTERS
Volume 21, Issue 11, Pages 1361-1365Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.cclet.2010.05.014
Keywords
Molecular imprinting; Conjugated enzyme; Sorbent assay; Parathion; Water sample
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Funding
- Excellent Young Teacher Fund of Anhui Province, China [2009SQRZ105]
- Anhui University of Architecture [QD20090905]
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A rapid, simple, and reliable competitive molecular imprinted polymer sorbent assay (MIPSA) was developed and validated for measurement of parathion in water samples. This assay employed a molecular imprinted polymer (MIP) that was synthesized with non-covalent imprinting method as capture reagent and p-aminoparathion conjugate of horseradish peroxidase (para-HRP) as an enzyme label. The assay depended on a competitive binding reaction between the enzyme conjugate and analyte for the binding sites of the MIP. The optimized analysis conditions of 10 ng mL(-1) para-HRP and 10 mg mL(-1) polymer were found. The assay was acceptable to detect parathion in water samples under the optimized conditions, with a limit of detection of 50 ng mL(-1). Mean analytical recoveries of added parathion in water samples ranged from 101.2% to 105%. The precision of the assay was satisfactory; relative standard deviation ranged from 4.3% to 6%. (C) 2010 Jian She Tang. Published by Elsevier B.V. on behalf of Chinese Chemical Society. All rights reserved.
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