Journal
CHINESE CHEMICAL LETTERS
Volume 21, Issue 3, Pages 346-348Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.cclet.2009.11.043
Keywords
Gold nanoparticles; Tyrosinase; Catechol; Fluorescence quenching
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Funding
- National Natural Science Foundation of China [20875059]
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The determination method of catechol by fluorescence quenching was developed. The assay was based on the combination of the unique property of gold nanoparticles with tyrosinase enzymatic reaction. In the presence of tyrosinase, the fluorescence of gold nanoparticles was quenched by catechol which can be employed to detect catechol. Under the optimal conditions, a linear range 5.0 x 10(-7)-1.0 x 10(-3) mol L-1 and a detection limit 1.0 x 10(-7) mol L-1 of catechol were obtained. o-Quinone intermediate produced from the enzymatic catalyzed oxidation of catechol was considered to play the main role in the fluorescence quenching. (C) 2009 Chuan Dong. Published by Elsevier B.V. on behalf of Chinese Chemical Society. All rights reserved.
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