Journal
CHINESE CHEMICAL LETTERS
Volume 21, Issue 4, Pages 472-475Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.cclet.2009.12.005
Keywords
O-Phenylenediamine; Abscisic acid; Immunosensor
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Funding
- National Natural Science Foundation of China [90817101, 30670190, 3060049]
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An impedance immunosensor based on O-phenylenediamine modified gold electrode for the determination of phytohormone abscisic acid (ABA) was proposed. The operating pH, absorption time, absorption temperature and concentration of anti-ABA antibody were investigated to optimize the analytical performance. The calibration curve for the determination of ABA was obtained from this impedance immunosensor under optimal conditions. The results showed that the detection limit at about 1 ng/mL in the range of 10-5000 ng/mL. (C) 2009 Lang Tao Xiao. Published by Elsevier B.V. on behalf of Chinese Chemical Society. All rights reserved.
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