Journal
CHINESE CHEMICAL LETTERS
Volume 20, Issue 9, Pages 1103-1106Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.cclet.2009.04.022
Keywords
Metabonomics; Fatty acids; Quantification; GC-MS; PLS-DA
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Funding
- National Basic Research Development Program of China [2005CB523503]
- National Natural Science Foundations of China [90709045, 20805026]
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A new metabonomics method was developed for simultaneous qualitative and quantitative analysis on esterified and nonesterified fatty acids (EFAs and NEFAs) in plasma. Merely 10 mu L of plasma was required. The pretreatment of the sample was simple without disposing the protein. After simple extraction and derivation, 15 FAs in plasma were precisely quantified. Gas chromatography tandem mass spectrometry (GC-MS) was used in the study and the quantities of the analytes, which varied in abundance over three orders of magnitude. The established method showed good accuracy. The curve correlation coefficients were all above 0.997. The RSDs of precision for all compounds were below 15% and recoveries were all between 80 and 110%. PLS-DA modal was used in the data management. (C) 2009 Guo An Luo. Published by Elsevier B.V. on behalf of Chinese Chemical Society. All rights reserved.
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