Journal
CHIMIA
Volume 63, Issue 11, Pages 714-720Publisher
SWISS CHEMICAL SOC
DOI: 10.2533/chimia.2009.714
Keywords
Excited state proton transfer; Imaging; Photoinduced electron transfer; Trace metals; X-ray fluorescence microscopy
Categories
Funding
- National Institutes of Health [GM067169, GM072021, K68096]
- NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES [R01DK068096] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [P20GM072021, R01GM067169] Funding Source: NIH RePORTER
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Metal-responsive fluorescent indicators are powerful tools for visualizing trace metals with subcellular resolution. By taking advantage of the diverse photophysical properties of organic fluorophores, metal ion-selective fluorescent indicators have been rationally designed and tailored towards cellular applications. This review summarizes challenges associated with the probe design and describes recent efforts in our research group in developing selective and sensitive reagents for the detection of zinc and copper in mammalian cells.
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