Journal
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
Volume 54, Issue 45, Pages 13245-13248Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.201505712
Keywords
biosensors; immobilization; odorant-binding protein; olfaction; reduced graphene oxide
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Funding
- European Science Foundation (ESF) [10-EuroBioSAS-FP-005]
- FFG within the comet program
- government of Lower Austria
- government of Upper Austria
- Austrian Science Fund (FWF) [I 681] Funding Source: researchfish
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An olfactory biosensor based on a reduced graphene oxide (rGO) field-effect transistor (FET), functionalized by the odorant-binding protein14 (OBP14) from the honey bee (Apis mellifera) has been designed for the insitu and real-time monitoring of a broad spectrum of odorants in aqueous solutions known to be attractants for bees. The electrical measurements of the binding of all tested odorants are shown to follow the Langmuir model for ligand-receptor interactions. The results demonstrate that OBP14 is able to bind odorants even after immobilization on rGO and can discriminate between ligands binding within a range of dissociation constants from K-d=4M to K-d=3.3mM. The strongest ligands, such as homovanillic acid, eugenol, and methyl vanillate all contain a hydroxy group which is apparently important for the strong interaction with the protein.
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