Journal
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
Volume 54, Issue 33, Pages 9614-9617Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.201502920
Keywords
cell mimic; liposomes; protein-ligand interactions; vesicles
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Funding
- Dutch Science Foundation (NWO)
- Dutch Ministry of Education, Culture and Science [024.001.035]
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External small-molecule triggers were used to reversibly control dynamic protein-ligand interactions in giant vesicles. An alcohol dehydrogenase was employed to increase or decrease the interior pH upon conversion of two different small-molecule substrates, thereby modulating the pH-sensitive interaction between a Ni-NTA ligand on the vesicle membrane and an oligohistidine-tagged protein in the lumen. By alternating the small-molecule substrates the interaction could be reversed.
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