Journal
CHEMSUSCHEM
Volume 7, Issue 11, Pages 3164-3171Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/cssc.201402465
Keywords
biocatalysis; biomass; cleavage reactions; lignins; enzymes
Funding
- Excellence Initiative of the German Research Foundation
- DFG training group 1166 BioNoCo (Biocatalysis in Non-conventional Media)
Ask authors/readers for more resources
Microbial -etherases, which selectively cleave the -O-4 aryl ether linkage present in lignin, hold great promise for future applications in lignin valorization. However, very few members have been reported so far and little is known about these enzymes. By using a database mining approach, four novel bacterial -etherases were identified, recombinantly produced in Escherichia coli, and investigated together with known -etherases in the conversion of various lignin and non-lignin-type model compounds. The resulting activities revealed the significant influence of the substituents at the phenyl ring adjacent to the ether bond. Finally, -etherase activity on polymeric substrates, measured by using a fluorescently labeled synthetic lignin, was also proven; this underlined the applicability of the enzymes for the conversion of lignin into renewable chemicals.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available