Journal
CHEMPHYSCHEM
Volume 15, Issue 11, Pages 2240-2246Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/cphc.201402002
Keywords
fluorescence; fluorescent probes; live cells; quantitative imaging; super-resolution imaging
Funding
- National Institute of Health [R00GM087810]
- University of Michigan
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Far-red organic fluorophores commonly used in traditional and super-resolution localization microscopy are found to contain a fluorescent impurity with green excitation and near-red emission. This near-red fluorescent impurity can interfere with some multicolor stochastic optical reconstruction microscopy/photoactivated localization microscopy measurements in live cells and produce subtle artifacts in chemically fixed cells. We additionally describe alternatives to avoid artifacts in super-resolution localization microscopy.
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