Journal
CHEMPHYSCHEM
Volume 15, Issue 16, Pages 3460-3465Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/cphc.201402509
Keywords
biomimetic materials; lipid bilayers; lipids; molecular recognition; phase behaviour
Funding
- European Research Council [259183]
- Council for Chemical Sciences of the Netherlands Organization for Scientific Research (NWO-CW) [700.58.443]
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Tuneable and stable surface-chemical gradients in supported lipid bilayers (SLBs) hold great promise for a range of applications in biological sensing and screening. Yet, until now, no method has been reported that provides temporal control of SLB gradients. Herein we report on the development of locked-in SLB gradients that can be tuned in space, time and density by applying a process to control lipid phase behaviour, electric field and temperature. Stable gradients of charged Texas-Red-, serine-or biotin-terminated lipids have been prepared. For example, the Texas-Red surface density was varied from 0 to 2 mol%, while the length was varied between several tens to several hundreds of microns. At room temperature the gradients are shown to be stable up to 24 h, while at 60 degrees C the gradients could be erased in 30 min. Covalent and non-covalent chemical modification of the gradients is demonstrated, for example, by FITC, hexahistidine-tagged proteins, and SAv/biotin. The amenability to various (bio) chemistries paves the way for novel SLB-based gradients, useful in sensing, high-throughput screening and for understanding dynamic biological processes.
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