Journal
CHEMPHYSCHEM
Volume 15, Issue 4, Pages 721-726Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/cphc.201300802
Keywords
cathodoluminescence; electron microscopy; high spatial resolution; live-cell imaging; multi-color imaging
Funding
- Grants-in-Aid for Scientific Research [23360031, 13J04082] Funding Source: KAKEN
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Multi-color, high spatial resolution imaging of fluorescent nanodiamonds (FNDs) in living HeLa cells has been performed with a direct electron-beam excitation-assisted fluorescence (D-EXA) microscope. In this technique, fluorescent materials are directly excited with a focused electron beam and the resulting cathodoluminescence (CL) is detected with nanoscale resolution. Green- and red-light-emitting FNDs were employed for two-color imaging, which were observed simultaneously in the cells with high spatial resolution. This technique could be applied generally for multi-color immunostaining to reveal various cell functions.
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