Journal
CHEMPHYSCHEM
Volume 13, Issue 4, Pages 973-981Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/cphc.201100743
Keywords
gold; nanostructures; silver; single molecules; super-resolution microscopy
Funding
- Fonds voor Wetenschappelijk Onderzoek FWO [G.0402.09, G0413.10, G0181.10, G0697.11, G0197.11]
- K.U. Leuven Research Fund [GOA 2011/03, CREA2009, IDO/07/010]
- Federal Science Policy of Belgium [IAP-VI/27, T2009.05]
- Hercules foundation [HER/08/21]
- Flemish government [CASAS METH/08/04]
- EC Marie-Curie ITN-SUPERIOR [PITN-GA-2009-238177]
- National Natural Science Foundation of China, NSFC [21173253]
- Ministry of Education of Spain
- JST PRESTO
- ERC [280064]
- European Research Council (ERC) [280064] Funding Source: European Research Council (ERC)
Ask authors/readers for more resources
Photoactivation localization microscopy (PALM) was applied to study surface-enhanced fluorescence (SEF) on metal nanostructures (SEF-PALM). The detection of fluorescence from individual single molecules can be used to image the point-spread-function and spatial distribution of the fluorescence emitted in the vicinity of a metal surface. Due to the strong scattering effect, the angular distribution of the fluorescence is altered by metals, resulting in a spatial shift of fluorescence spots with respect to the metal nanostructures, and has to be taken into account in the analysis. SEF-PALM can be used to discriminate effects of labelling density when estimating the enhancement factor in SEF. Furthermore, nanostructures with sizes below the diffraction limit can be resolved using this technique. SEF-PALM is established as a powerful tool to study plasmon-mediated phenomena on metal nanostructures.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available