Journal
CHEMPHYSCHEM
Volume 10, Issue 9-10, Pages 1511-1516Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/cphc.200900113
Keywords
biophysics; lipids; motor protein; particle tracking; single-molecule studies
Funding
- NIH
- NSF
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We report a label free assay for simultaneous optical manipulation and tracking of endogenous lipid droplets as actively transported cargoes in a living mammalian cell with sub milli-second time resoultion. Using an EM-Cd camera as a highly sensitive quadrant detector. we can detect steps of dynein- and kinesin-driven cargoes under known force loads. We can distinguish single and multiple motor-driven cargoes and show that the stall forces for inward and outward transported cargoes are similar By combining the stall force observable with the ability to detect individual steps, we can characterize kinesin and dynein-driven active transport in difference force regimes.
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