Journal
CHEMPHYSCHEM
Volume 9, Issue 5, Pages 707-712Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/cphc.200700839
Keywords
dark-field microscopy; gold nanoparticles; motor protein; organelle transport; particle tracking
Funding
- NIGMS NIH HHS [P20 GM072069] Funding Source: Medline
Ask authors/readers for more resources
The study of cellular processes such as organelle transport often demands particle tracking with microsecond time-resolution and nanometer spatial precision, posing significant challenges to existing tracking methods. Here, we have developed a novel strategy for two-dimensional tracking of gold nanoparticles (GNPs) with 25 mu s time resolution and similar to 1.5 nm spatial precision, by using a quadrant photodiode to record the positions of GNPs in an objective-type dark-field microscope. In combination with a feedback loop, this technique records long, high time-resolution and spatial precision trajectories of endocytosed GNPs transported by the molecular motors kinesin and dynein in a living cell. In the full range of orgonelle velocities (0-8 mu m s(-1)), we clearly resolve the individual 8 nm steps of cargoes carried by kinesin, and the 8, 12 16, 20, and 24 nm steps of those carried by dynein. These experiments yield new information about molecular motor stepping in living cells.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available