Journal
CHEMOSPHERE
Volume 109, Issue -, Pages 150-156Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.chemosphere.2014.01.073
Keywords
Biodegradation; Biodiesel; Inoculum; Microbial growth phases; Lag phase
Categories
Funding
- USGS NIWR program
- FTA via the Integrated Concepts and Research Corporation Cold-Weather Fischer-Tropsch Fuels Demonstration project [AK-26-7005]
- INRA fellowship
- UAF
Ask authors/readers for more resources
To achieve effective bioremediation within short warm seasons of cold climates, microbial adaptation periods to the contaminant should be brief. The current study investigated growth phases for soil spiked with diesel, Syntroleum, or fish biodiesel, using microbial inocula adapted to the specific substrates. For modeling hydrocarbon degradation, multi-phase first order kinetics was assumed, comparing linear regression with nonlinear parameter optimization of rate constants and phase durations. Lag phase periods of 5 to >28 d were followed by short and intense exponential growth phases with high rate constants (e.g. from k(Fish) = 0.0013 +/- 0.0002 to k(Syntr) = 0.015 +/- 0.001 d(-1)). Hydrocarbon mineralization was highest for Syntroleum contamination, where up to three times higher cumulative CO2 production was achieved than for diesel fuel, with fish biodiesel showing initially the slowest degradation. The amount of hydrocarbons recovered from the soil by GC-MS decreased in the order fish biodiesel > diesel > Syntroleum. During initial weeks, biodegradation was higher for microbial inocula adapted to a specific fuel type, whereby the main effect of the inoculum was to shorten the lag phase duration; however, the inoculum's importance diminished after daily respiration peaked. In conclusion, addition of an inoculum to increase biodegradation rates was not necessary. (C) 2014 Elsevier Ltd. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available