Journal
CHEMOSPHERE
Volume 92, Issue 2, Pages 225-232Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.chemosphere.2013.01.089
Keywords
Cytogenotoxicity; Decolorization; Phytoremediation; Reactive Blue 160; Tagetes patula L
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Funding
- Department of Biotechnology (DBT), New Delhi, India
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Tagetes patula is an annual flowering plant belonging to family Asteraceae. The present study deals with in vitro decolorization and remediation of a textile dye Reactive Blue 160 by T. patula. There was considerable (similar to 90%) decolorization of the dye within 4 d of incubation, as confirmed by UV-vis, HPLC and FTIR analysis. The enzymes responsible for the remediation were lignin peroxidase, tyrosinase, laccase and NADH-DCIP reductase which were found in root tissues of the plantlets. GC-MS analysis of the products revealed formation of six metabolites such as sodium benzenesulfonate, 6-chloro 1,3,5-triazine-2,4-diamine, disodium benzene-1,4-disulfonate, sodium 3-amino-4-hydroxybenzenesulfonate, 1-phenylmethanediamine and sodium 4-amino-3-carboxybenzenesulfonate after phytoremediation of Reactive Blue 160. Based on the FTIR and GC-MS results, the possible pathway for the biodegradation of Reactive Blue 160 has been traced. The non-toxic nature of the degraded products was confirmed by performing cytogenotoxicity tests on root tip cells of growing Allium cepa. (C) 2013 Elsevier Ltd. All rights reserved.
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