Journal
CHEMOSPHERE
Volume 85, Issue 7, Pages 1130-1138Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.chemosphere.2011.07.053
Keywords
Heavy metal-resistant bacterial endophytes; Solanum nigrum L.; Phylogenetic analysis; Phytoremediation
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Funding
- National Science Fund for Distinguished Young Scholars [50725825]
- National Natural Science Foundation of China [50830301]
- Hunan Provincial Natural Science Foundation of China [10JJ5051]
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This study investigates the heavy metal-resistant bacterial endophytes of Cd-hyperaccumulator Solanum nigrum L grown on a mine tailing by using cultivation-dependent technique. Thirty Cd-tolerant bacterial endophytes were isolated from roots, stems, and leaves of S. nigrum L and classified by amplified ribosomal DNA-restriction analysis into 18 different types. Phylogenetic analysis based on 16S rDNA sequences showed that these isolates belonged to four groups: Actinobacteria (43%), Proteobacteria (23%), Bacteroidetes (27%) and Firmicutes (7%). All the isolates were then characterized for their plant growth promoting traits as well as their resistances to different heavy metals: and the actual plant growth promotion and colonization ability were also assessed. Four isolates were re-introduced into S. nigrum L under Cd stress and resulted in Cd phytotoxicity decrease, as dry weights of roots increased from 55% to 143% and dry weights of above-ground from 64% to 100% compared to the uninoculated ones. The total Cd accumulation of inoculated plants increased from 66% to 135% (roots) and from 22% to 64% (above-ground) compared to the uninoculated ones. Our research suggests that bacterial endophytes are a most promising resource and may be the excellent candidates of bio-inoculants for enhancing the phytoremediation efficiency. (C) 2011 Elsevier Ltd. All rights reserved.
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