Journal
CHEMOSPHERE
Volume 79, Issue 11, Pages 1084-1088Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.chemosphere.2010.03.018
Keywords
Chlorophenoxy acids; Clofibric acid; Microbial degradation; Carbon source
Categories
Funding
- Fonds quebec-ois de la recherche sur la nature et les technologies du Quebec
- Natural Sciences and Engineering Research Council of Canada (NSERC)
- Eugenie Ulmer Lamothe Fund
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Pseudomonas putida, Aspergillus niger, Bacillus subtilis, Pseudomonas flourescens, Sphingomonas herbicidovorans and Rhodococcus rhodochrous growing on glucose in a medium containing one of three chlorophenoxy acids at a concentration of 0.1 g L-1 (clofibric acid, (R)-2-(4-chloro-2-methylphenoxy)propionic acid (mecoprop or MCPP) and 4-chloro-2-methylphenoxyacetic acid (MCPA)) degraded these compounds to varying degrees: from nonmeasurable to almost complete removal. These results with the addition of glucose (2,5 g L-1) as an easy to use carbon source indicated the formation of metabolites different from results reported in the literature for growth studies in which the chlorophenoxy acid was the sole carbon source. The metabolite, 4-chloro-2-methylphenol, which had been reported previously, was only observed in trace amounts for MCPP and MCPA in the presence of S. herbicidovorans and glucose. In addition, three other compounds (M1, M3 and M4) were observed. It is suggested that these unidentified metabolites resulted from ring opening of the metabolite 4-choloro-2-methylphenol (M2). The rate of biodegradation of the chlorophenoxy acids was influenced by the degree of steric hindrance adjacent to the internal oxygen bond common to all three compounds. The most hindered compound, clofibric acid, was converted to ethyl clofibrate by R. rhodochrous but was not degraded by any microorganisms studied. The more accessible internal oxygen bonds of the other two chlorophenoxy acids, MCPP and MCPA, were readily broken by S. herbicidovorans. (C) 2010 Elsevier Ltd. All rights reserved.
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