Effects of endosulfan and ethanol on the reproduction of the snail Biomphalaria tenagophila: A multigeneration study

Endosulfan (END) is an insecticide used in agriculture and as a wood preservative. Since END is practically insoluble in water, ethanol (ETCH) is often employed as a carrier solvent to spike it in the test medium in aquatic toxicity assays. In this study were investigated the effects of END and ETOH on the reproduction of the freshwater snail Biomphalaria tenagophila exposed over three successive generations. END (0, 0.001. 0.01, 0.1 mg L-1) was dissolved in the medium water using ETOH (up to 19.8 mg L-1) as carrier solvent. ETOH (19.8, 198, 1980 mg L-1) alone was tested as well. Adult snails (F-0-generation) were exposed to END and ETCH for 8 weeks. The F-1-generation continued to be exposed from embryo to reproductive maturity, while their descendants (F-2) were exposed until day 10 after spawning. Effects on the fecundity (8-week production of eggs and egg-masses) of mature F-0 and F-1 snails were evaluated. Developmental toxicity was investigated in F-1 and F-2 embryos. END at the highest level tested (0.1 mg L-1) inhibited egg production by F-0 and F-1 snails. ETOH at levels >= 198 mg L-1 also reduced fecundity of F-0 and F-1 an effect that was apparently aggravated by exposure over successive generations. END 0.1 mg L-1 increased mortality and malformations and decreased hatching among F-1 embryos. ETOH drastically reduced the proportion of hatchings among F-2 embryos. The study-derived NOECs (no-observed-effect-concentrations) for END was 0.01 mg L-1 (reduction in fecundity), and for ETOH were 19.8 mg L-1 for reduction in fecundity and <19.8 mg L-1 for developmental toxicity (hatching retardation). (C) 2008 Elsevier Ltd. All rights reserved.