Journal
CHEMISTRY-AN ASIAN JOURNAL
Volume 9, Issue 7, Pages 1777-1781Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/asia.201402043
Keywords
biosensors; biothiols; excited state intramolecular proton transfer; fluorescent probes; imaging agents; NIR emission
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Funding
- National Science Foundation of China [21172071, 21190033, 21372083]
- Fundamental Research Funds for the Central Universities
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Background autofluorescence from biological systems generally reduces the sensitivity of a fluorescent probe for imaging biological targets. Addressing this challenge requires the development of fluorescent probes that produce emission in the near-infrared region. Herein, we report the design and synthesis of a fluorescent probe that generates an NIR emission with a large Stokes shift upon the selective response to Cys over Hcy and GSH. The probe is designed to consist of two Cys-sensing sites, an acrylate ester and an aldehyde installed ortho to each other. The reaction of the probe with Cys triggers an excited state intramolecular proton transfer process upon photo-excitation, thereby producing an NIR emission with a large Stokes shift. Accordingly, this probe hold great promise for the selective detection of Cys in biological systems. We further demonstrate the capacity of this probe for Cys imaging in living cells.
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