Journal
CHEMISTRY-AN ASIAN JOURNAL
Volume 7, Issue 11, Pages 2678-2682Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/asia.201200362
Keywords
aggregation; carbohydrates; imaging agents; nanoparticles; quantum dots
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Funding
- Japan Science and Technology Agency (Evolutionally venture program) [V07-05]
- Japan Science and Technology Agency (CREST)
- Japan Science and Technology Agency (Research for Promoting Technological Seeds) [16-057]
- Japan Ministry of Health and Welfare
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Sugar chains are important molecules in cellular recognition and signaling, and quantum dots (QDs) are a very powerful tool for in vitro and in vivo imaging. Herein, we report the preparation of stable sugar-chain-immobilized fluorescent nanoparticles (SFNPs) and their application to the analysis of sugar-chainprotein interactions and cellular imaging. SFNPs were easily prepared by mixing CdTe/CdS core/shell QDs with our previously developed sugar-chainligand conjugates. The obtained SFNPs were very stable and could be stored for several months. In the binding analysis, beta-galactose- and a-glucose-immobilized SFNPs were specifically interacted with Ricinus communis agglutinin I and concanavalin A, respectively, and made into aggregates. The binding interaction was detected visually, fluorescently, or both. In the experiment for cellular imaging, it was found that SFNPs were predominantly taken up by human hepatocyto carcinoma cells (HepG2), suggesting the possible usage of our designed SFNPs for various biochemical analyses of sugar chains.
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