Journal
CHEMISTRY-AN ASIAN JOURNAL
Volume 6, Issue 10, Pages 2730-2737Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/asia.201100411
Keywords
cell encapsulation; cycloaddition; gels; rheology; ring-opening polymerization
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Funding
- National Institutes of Health [R01AR055615, R21AR056866]
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Strategies to encapsulate cells in cytocompatible three-dimensional hydrogels with tunable mechanical properties and degradability without harmful gelling conditions are highly desired for regenerative medicine applications. Here we reported a method for preparing poly(ethylene glycol)-co-polycarbonate hydrogels through copper-free, strain-promoted azide-alkyne cycloaddition (SPAAC) click chemistry. Hydrogels with varying mechanical properties were formed by clicking azido-functionalized poly(ethylene glycol)-co-polycarbonate macromers with dibenzocyclooctyne-functionalized poly(ethylene glycol) under physiological conditions within minutes. Bone marrow stromal cells en-capsulated in these gels exhibited higher cellular viability than those encapsulated in photo-cross-linked poly(ethylene glycol) dimethacrylate. The precise control over the macromer compositions, cytocompatible SPAAC cross-linking, and the degradability of the polycarbonate segments make these hydrogels promising candidates for scaffold and stem cell assisted tissue repair and regeneration.
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