Journal
CHEMISTRY-AN ASIAN JOURNAL
Volume 6, Issue 8, Pages 2027-2032Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/asia.201100156
Keywords
calcium; fluorescence; nonlinear spectroscopy; microscopy; two-photon probe
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Funding
- Korean Government [2010-0018921]
- Ministry of Education, Science and Technology [2010-0020209]
- BK21 scholarship
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We report a two-photon (TP) probe (ACaLN) for near-membrane Ca2+ that shows a 13-fold TP excited fluorescence (TPEF) enhancement in response to Ca2+, dissociation constants (K-d(TP)) of (1.9 +/- 0.2) mu m, pH-insensitivity at the biologically relevant pH, and can detect near-membrane Ca2+ in live cells for more than 1500 s and in living tissues at 120 mu m depth without interference from other metal ions. Comparison with existing probes provides a useful strategy for the design of efficient TP probes for the near-membrane metal ions.
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