Journal
CHEMISTRY-AN ASIAN JOURNAL
Volume 5, Issue 5, Pages 1185-1191Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/asia.200900542
Keywords
core-shell nanoparticles; enrichment; glycopeptides; mass spectrometry; polymerization
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Funding
- National Science and Technology Key Project of China [2007CB914100, 2008ZX10207, 2009CB825607, 2006AA02A308]
- National Natural Science Foundation of China [20735005, 20875016, 30672394, 20873029]
- Ph.D. Programs Foundation of the Ministry of Education of China [200802460011]
- NCET
- Shanghai Projects [08DZ2293601, 09QA1400400, B109]
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The core-shell boronic-acid functionalized nanoparticles SnO2@Poly(HEMA-co-St-co-VPBA) are designed for selectively enriching glycopeptides, followed by matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) analysis. Such 60 nm sized core-shell nanoparticles are prepared by means of copolymerization between 2-hydroxyethyl methacrylate (HEMA) grafted on SnO2 nanoparticles, styrene, and 4-vinylphenylboronic acid (VPBA). All of the synthesis procedures are completed within 3 h. Cyclic boronate esters form between boronic-acid groups on the polymer chains and cis-diol groups on glycopeptides, and thus almost all intact glycopeptides from low-abundant horseradish peroxidase (HRP) and bovine asialofetuin (ASF) are enriched with high selectivity and efficiency. After enrichment, both intact N- and O-glycopeptides are characterized by multistage MS. Furthermore, we successfully apply this method to the human serum sample for characterizing the target glycoproteins haptoglobin and alpha-l-acid-glycoprotein. The present selective enriching method followed by multistage-MS analysis is proven to be a good choice for routine glycopeptide characterization.
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