Journal
CHEMISTRY-AN ASIAN JOURNAL
Volume 5, Issue 3, Pages 581-588Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/asia.200900386
Keywords
boronic acid; fluorophore; nucleoside; quencher; receptor
Categories
Funding
- Royal Society [2007/R2]
- Leverhulme Trust [JSF F/00351/P]
- University of Birmingham
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Fluorescein isothiocyanate is treated with 3-aminophenylboronic acid to provide a fluorescently tagged boronic acid derivative which is used to assess Forster resonance energy transfer (FRET) quenching upon boronate ester formation with a series of bespoke diol appended quenchers. Fluorescence spectroscopy comparison of quenching efficiency between treatment of fluorescein and its boronic acid appended congener with quencher appended diol reveals boronate ester formation (covalently linked) to be the more efficient regime and from the panel of quenchers which also included nucleosides.
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