Journal
CHEMISTRY-AN ASIAN JOURNAL
Volume 5, Issue 2, Pages 301-308Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/asia.200900297
Keywords
benzothiadiazole; biosensors; click chemistry; FRET; polymers
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Funding
- National Univeisity of Singapore [R-279-000-234-123]
- Ministry of Education [R-279-000-255-112]
- Temasek Defense Systems Institute [R-279-000-268-422, R-279-000-268-592, R-279-000-268-212]
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A neutral polyfluorene derivative that contains 20 mol% 2,1,3-benzothiadiazole (BT) is synthesized by Suzuki cross-coupling polymerization. A cationic conjugated polymer A and an alpha-mannose-bearing polymer B are subsequently obtained through different post-polymerization methods. As it result of the charged pendant groups or sugar-bearing groups attached to the polymer side chains, both A and B show good water-solubility. The titration of Concanavalin A (Con A) into polymer aqueous solution leads to different fluorescent responses for polymers A and B. Polymer A does not show any obvious fluorescence change upon interaction with Con A. whereas polymer B shows fluorescence increase in BT emission intensity when Con A is added. This is because Of the Specific interaction between alpha-mannose and Con A, which induces polymer aggregation, and then facilitates energy transfer from the phenylene-fluorene segments to the BT units. A practical calibration curve ranging front 1 nM to 250 nM is obtained by correlating the changes in BT emission intensity with Con A concentration. The advantage of polymer B-based Con A macromolecular probe is that it shows signal increase upon Con A recognition, which is significantly different from other conjugated polymer-based fluorescence quenching assays.
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