Journal
CHEMISTRY-AN ASIAN JOURNAL
Volume 4, Issue 12, Pages 1802-1808Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/asia.200900357
Keywords
drug discovery; liquid chromatography; magnetic beads; natural products; proteins
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Funding
- Ministry of Education, Culture, Sports. Science and Technology, Japan
- Uehara Memorial Foundation
- Mitsubishi Chemical Corporation Fund
- Hamaguchi Foundation for the Advancement of Biochemistry
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A highly efficient screening method for naturally occurring products that bind to a specific target protein was demonstrated by using hVDR magnetic beads. The native ligand 1 alpha,25(OH)(2) VD3 (1) was selectively bound by hVDR magnetic beads when present in a mixture of natural compounds. Furthermore, this method was shown to be applicable to the identification of natural products that interact with a specific protein immobilized on the beads from an extract of a natural resource. Two new natural compounds were isolated by this method. This approach will be helpful for the discovery of novel, naturally occurring products that bind to specific target proteins. This method has the further advantages that it can identify the HPLC peak corresponding to the target compound for isolation, as well as provide important UV, CD, or MS profile information.
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