Journal
CHEMISTRY-AN ASIAN JOURNAL
Volume 3, Issue 11, Pages 1942-1948Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/asia.200800152
Keywords
DNA; electrochemiluminescence; enzyme catalysis; platinum nanoparticles; polymers
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Funding
- National Natural Science Foundation of China [20675078, 90713022, 20735003, 2007CB714500]
- Chinese Academy of Sciences [KJCX2.YW.H11]
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Polyethyleneimine-functionalized platinum nanoparticles (PtNPs) with excellent electrochemiluminescence (ECL) properties were synthesized and applied to the amplified analysis of biomolecules. These particles were prepared at room temperature, with hyperbranched polyethyleneimine (HBPEI) as the stabilizer. The UV/Vis absorption spectra and transmission electron microscopy images clearly confirmed the formation of monodisperse PtNPs. Such particles proved to possess high stability against salt-induced aggregation, enabling them to be employed even under high-salt conditions. Owing to the existence of many tertiary amine groups, these particles exhibited excellent ECL behavior in the presence of tris(2.2'-bipyridyl)ruthenium(II). An HBPEI-coated particle possessed an ECL activity that was at least 60 times higher than that of a tripropylamine molecule. Furthermore, these particles could be immobilized on the 3-aminopropyltriethoxysilane-treated quartz substrates to amplify the binding sites for carboxyl groups. Through this approach, PtNPs were applied to the amplified analysis of the hemin/G-quadruplex DNAzyme by using the luminol/H2O2 chemiluminescence method.
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