Journal
CHEMISTRY-A EUROPEAN JOURNAL
Volume 19, Issue 7, Pages 2276-2283Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/chem.201203641
Keywords
carbon dots; cell imaging; luminescence; nitrogen; upconversion
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Funding
- National Basic Research Program of China (973 Program) [2011CB707703, 2012CB910601]
- National Natural Science Foundation of China [81071260, 21075068]
- Tianjin Science Technology Research Funds [10JCZDJC17000, 11JCZDJC22200]
- Fundamental Research Funds for the Central Universities
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Carbon dots (Cdots) are an important probe for imaging and sensing applications because of their fluorescence property, good biocompatibility, and low toxicity. However, complex procedures and strong acid treatment are often required and Cdots suffer from low photoluminescence (PL) emission. Herein, a facile and general strategy using carbonization of precursors and then extraction with solvents is proposed for the preparation of nitrogen-doped Cdots (N-Cdots) with 3-(3,4-dihydroxyphenyl)-L-alanine (L-DOPA), L-histidine, and L-arginine as precursor models. After they are heated, the precursors become carbonized. Nitrogen-doped Cdots are subsequently extracted into N,N-dimethylformamide (DMF) from the carbogenic solid. A coreshell structure of Cdots with a carbon core and the oxygen-containing shell was observed. Nitrogen has different forms in N-Cdots and oxidized N-Cdots. The doped nitrogen and low oxidation level in N-Cdots improve their emission significantly. The N-Cdots show an emission with a nitrogen-content-dependent intensity and Cdot-size-dependent emission-peak wavelength. Imaging of HeLa cells, a human cervical cancer cell line, and HepG2 cells, a human hepatocellular liver carcinoma line, was observed with high resolution using N-Cdots as a probe and validates their use in imaging applications and their multicolor property in the living cell system.
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