Journal
CHEMISTRY-A EUROPEAN JOURNAL
Volume 18, Issue 52, Pages 16708-16715Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/chem.201202276
Keywords
apoptosis; cancer; oligosaccharides; peptides; polymers; protein-protein interactions
Categories
Funding
- DFG [Ra895-4-6, SFB 765, FOR 806, BR2443-5]
- Fonds der Chemischen Industrie
Ask authors/readers for more resources
Multivalent peptideoligosaccharide conjugates were prepared and used to investigate the multivalency effect concerning the activity of Bid-BH3 peptides in live cells. Dextran oligosaccharides were carboxyethylated selectively in the 2-position of the carbohydrate units and activated for the ligation of N-terminally cysteinylated peptides. Ligation through maleimide coupling was found to be superior to the native chemical ligation protocol. Monomeric Bid-BH3 peptides were virtually inactive, whereas pentameric peptide conjugates induced apoptosis up to 20-fold stronger at identical peptide concentrations. Comparison of lowly multivalent and highly multivalent peptide dextrans proved a multivalency effect in life cells which was specific for the BH3 peptide sequence.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available