Journal
CHEMISTRY-A EUROPEAN JOURNAL
Volume 18, Issue 21, Pages 6485-6492Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/chem.201104069
Keywords
bacterial recognition; carbohydrates; diamond; nanoparticles; surface chemistry
Categories
Funding
- Deutsche Forschungsgemeinschaft
- European Commission
Ask authors/readers for more resources
The detection and removal of bacteria, such as E. coli in aqueous environments by using safe and readily available means is of high importance. Here we report on the synthesis of nanodiamonds (ND) covalently modified with specific carbohydrates (glycoND) for the precipitation of type 1 fimbriated uropathogenic E. coli in solution by mechanically stable agglutination. The surface of the diamond nanoparticles was modified by using a DielsAlder reaction followed by the covalent grafting of the respective glycosides. The resulting glycoND samples are fully dispersible in aqueous media and show a surface loading of typically 0.1 mmol?g-1. To probe the adhesive properties of various ND samples we have developed a new sandwich assay employing layers of two bacterial strains in an array format. Agglutination experiments in solution were used to distinguish unspecific interactions of glycoND with bacteria from specific ones. Two types of precipitates in solution were observed and characterized in detail by light and electron microscopy. Only by specific interactions mechanically stable agglutinates were formed. Bacteria could be removed from water by filtration of these stable agglutinates through 10 mu m pore-size filters and the ND conjugate could eventually be recovered by addition of the appropriate carbohydrate. The application of glycosylated ND allows versatile and facile detection of bacteria and their efficient removal by using an environmentally and biomedically benign material.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available