Journal
CHEMISTRY-A EUROPEAN JOURNAL
Volume 18, Issue 7, Pages 2014-2020Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/chem.201103328
Keywords
lanthanides; microspheres; peptides; phosphate; rare-earth metals
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Funding
- National Natural Science Foundation of China (NNSFC) [20871083, 21171161]
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Rare-earth phosphate microspheres with unique structures were developed as affinity probes for the selective capture and tagging of phosphopeptides. Prickly REPO4 (RE=Yb, Gd, Y) monodisperse microspheres, that have hollow structures, low densities, high specific surface areas, and large adsorptive capacities were prepared by an ion-exchange method. The elemental compositions and crystal structures of these affinity probes were confirmed by energy-dispersive spectroscopy (EDS), powder X-ray diffraction (XRD), and Fourier-transform infrared (FTIR) spectroscopy. The morphologies of these compounds were investigated using scanning electron microscopy (SEM), transmission electron microscopy (TEM), and nitrogen-adsorption isotherms. The potential ability of these microspheres for selectively capturing and labeling target biological molecules was evaluated by using protein-digestion analysis and a real sample as well as by comparison with the widely used TiO2 affinity microspheres. These results show that these porous rare-earth phosphate microspheres are highly promising probes for the rapid purification and recognition of phosphopeptides.
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