Journal
CHEMISTRY-A EUROPEAN JOURNAL
Volume 18, Issue 51, Pages 16383-16392Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/chem.201200407
Keywords
DNA; gene therapy; non-viral vector; polypyridyl; ruthenium
Categories
Funding
- Department of Science and Technology (DST), New Delhi, India [SR/S5/BC-25/2006, SR/FTP/CS-96/2007]
- University of Pune
Ask authors/readers for more resources
Complexes of the type [Ru(bxbg)2(N-N)]2+, where N-N denotes 2,2'-bipyridine (bpy) (1), 1,10-phenanthroline (phen) (2), dipyrido[3,2-d:2',3-f] quinoxaline (dpq) (3), and dipyrido[3,2-a:2',3'-c]phenazine (dppz) (4), incorporating bis(o-xylene)bipyridine-glycoluril (bxbg) as an ancillary molecular clip ligand, have been synthesized and characterized. These ruthenium(II) complexes of bis(o-xylene)bipyridine-glycoluril self-associate in water through specific molecular recognition processes to form polycationic arrays. These arrays containing electrostatic binders as well as intercalator ligands at micromolar doses rapidly condense free DNA into globular nanoparticles of various sizes. The DNA condensation induced by these complexes has been investigated by electrophoretic mobility assay, dynamic light scattering, and transmission electron microscopy. The cellular uptake of complexDNA condensates and the low cytotoxicity of these complexes satisfy the requirements of a gene vector.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available