Journal
CHEMISTRY-A EUROPEAN JOURNAL
Volume 18, Issue 25, Pages 7787-7792Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/chem.201200023
Keywords
cell recognition; endocytosis; integrin; mesoporous materials; RGD
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Funding
- US National Science Foundation [CHE-0809521]
- US Department of Energy, Ames Laboratory, Office of Basic Energy Sciences [DE-AC02-07CH11358]
- Division Of Chemistry
- Direct For Mathematical & Physical Scien [0809521] Funding Source: National Science Foundation
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Recent breakthrough research on mesoporous silica nanoparticle (MSN) materials has illustrated their significant potential in biological applications due to their excellent drug delivery and endocytotic behavior. We set out to determine if MSN, covalently functionalized with conformation specific bioactive molecules (either linear or cyclic RGD ligands), behave towards mammalian cells in a similar manner as the free ligands. We discovered that RGD immobilized on the MSN surface did not influence the integrity of the porous matrix and improved the endocytosis efficiency of the MSN materials. Through competition experiments with free RGD ligands, we also discovered a conformation specific receptorintegrin association. The interaction between RGD immobilized on the MSN surface and integrins plays an important role in endosome trafficking, specifically dictating the kinetics of endosomal escape. Thus, covalent functionalization of biomolecules on MSN assists in the design of a system for controlling the interface with cancer cells.
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