Journal
CHEMISTRY-A EUROPEAN JOURNAL
Volume 17, Issue 35, Pages 9651-9657Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/chem.201100177
Keywords
DNA damage; enzymes; oligonucleotides; spore photoproduct; UV irradiation
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Funding
- CiPSM
- [SFB 749]
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UV light is one of the major causes of DNA damage. In spore DNA, due to an unusual packing of the genetic material, a special spore photoproduct lesion (SP lesion) is formed, which is repaired by the enzyme spore photoproduct lyase (Spl), a radical S-adenosylmethionine (SAM) enzyme. We report here the synthesis and DNA incorporation of a DNA SP lesion analogue lacking the phosphodiester backbone. The oligonucleotides were used for repair studies and they were cocrystallized with a polymerase enzyme as a template to clarify the configuration of the SP lesion and to provide information about the base-pairing properties of the lesion. The structural analysis together with repair studies allowed us to clarify the identity of the preferentially repaired lesion diastereoisomer.
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