Journal
CHEMISTRY-A EUROPEAN JOURNAL
Volume 17, Issue 47, Pages 13274-13283Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/chem.201102300
Keywords
DNA; FRET; G-quadruplexes; mass spectrometry; transition metals
Categories
Funding
- Centre National de la Recherche Scientifique
- Institut Curie
- Agence Nationale de la Recherche (ANR) [ANR-09-BLAN- 0355]
- Conseil regional d' Aquitaine and Association pour la Recherche sur le Cancer (ARC)
- Fonds de la Recherche Scientifique-FNRS
- Agence Nationale de la Recherche (ANR) [ANR-09-BLAN-0355] Funding Source: Agence Nationale de la Recherche (ANR)
Ask authors/readers for more resources
Fifteen complexes of palladium, platinum, and copper, featuring five different N-donor tridentate (terpyridine-like) ligands, were prepared with the aim of testing their G-quadruplexDNA binding properties. The fluorescence resonance energy transfer melting assay indicated a striking positive effect of palladium on G-quadruplex DNA stabilization compared with platinum and copper, as well as an influence of the structure of the organic ligand. Putative binding modes (noncoordinative p stacking and base coordination) of palladium and platinum complexes were investigated by ESI-MS and UV/Vis spectroscopy experiments, which all revealed a greater ability of palladium complexes to coordinate DNA bases. In contrast, platinum compounds tend to predominantly bind to quadruplex DNA in their aqua form by noncoordinative interactions. Remarkably, complexes of [Pd(ttpy)] and [Pd(tMebip)] (ttpy=tolylterpyridine, tMebip=2,2'-(4-p-tolylpyridine-2,6-diyl)bis(1-methyl-1H-benzo[d]imidazole)) coordinate efficiently G-quadruplex structures at room temperature in less than 1 h, and are more efficient than their platinum counterparts for inhibiting the growth of cancer cells. Altogether, these results demonstrate that both the affinity for G-quadruplex DNA and the binding mode of metal complexes can be modulated by modifying either the metal or the organic ligand.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available