Journal
CHEMISTRY-A EUROPEAN JOURNAL
Volume 17, Issue 19, Pages 5311-5318Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/chem.201003393
Keywords
molecular recognition; nucleotides; sensors; solid-phase synthesis; supramolecular chemistry
Categories
Funding
- Deutsche Forschungsgemeinschaft (DFG)
- Fonds der Chemischen Industrie
- Studienstiftung des Deutschen Volkes
Ask authors/readers for more resources
The water-soluble tweezer receptor 1 with two symmetric peptidic arms, which are connected by an aromatic scaffold and contain lysine, phenylalanine, and a guanidinium-based anion-binding site as headgroup, has been synthesized. UV/Vis-derived Job plots show that the receptor forms 1:1 complexes with nucleotides and phosphate in buffered water at neutral pH. Binding constants have been determined by fluorescence and UV/Vis spectroscopy. All nucleotides tested were bound very efficiently, even in pure water, with binding constants between 10(4) and 10(5) M-1. Interestingly, all mononucleotides were bound much stronger than phosphate by a factor of at least 5 to 10. Furthermore 1 favors the binding of adenosine monophosphate (AMP) over adenosine diphosphate (ADP) and adenosine triphosphate (ATP), which is unprecedented for artificial nucleotide receptors reported so far. According to NMR spectroscopy and molecular modeling studies, the efficient binding is a result of strong electrostatic contacts supported by pi-pi interactions with the nucleobase within the cavity-shaped receptor.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available