Journal
CHEMISTRY-A EUROPEAN JOURNAL
Volume 17, Issue 2, Pages 641-648Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/chem.201001128
Keywords
antigens; biosensors; cancer; magnetic electrochemiluminescence; quantum dots
Categories
Funding
- National Natural Science Foundation of China [20975058]
- National Research Foundation for Postdoctoral Students [20090451288]
- National Basic Research Program of China [2010CB732404]
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Magnetic electrochemiluminescent Fe3O4/CdSe-CdS nanoparticle/polyelectrolyte nanostructures have been synthesized and used to fabricate an electrochemiluminescence (ECL) immunosensor for the detection of carcinoembryonic antigen (CEA). CEA is a protein used as a biomarker for several cancers; particularly, to monitor response to treatment in colon and rectal cancer patients. The nanocomposites can be easily separated and firmly attached to an electrode owing to their excellent magnetic properties. This represents a promising advantage for bioassay applications. More importantly, the nanostructures exhibit intense and stable ECL emissions in neutral solution, which makes them ideal for ECL immunosensing. The 3-aminopropyltriethoxysilane (APS) polyelectrolyte shell on the nanostructure surface not only enhances the intensity and stability of the ECL signal, but also acts as a crosslinker for immunosensor fabrication. A CEA antibody immobilized onto a nanocomposite/APS/electrode with gold nanoparticles comprises the ECL immunosensor. The principle of ECL detection for CEA is based on a change in steric hindrance after immunoreaction, which leads to a decrease in ECL intensity. A wide detection range (0.064 pg ml(-1)-10 ng ml(-1)) and low detection limit (0.032 pg ml(-1)) are achieved. The immunosensor is highly sensitive and selective, and exhibits excellent stability and good reproducibility. It thus has great potential for clinical protein detection. In particular, this approach uses a novel class of bifunctional nanocomposites that display both intense ECL and excellent magnetism, which renders them suitable for a large range of bioassay applications.
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