Journal
CHEMISTRY-A EUROPEAN JOURNAL
Volume 16, Issue 36, Pages 11115-11120Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/chem.201000574
Keywords
analytical methods; cell recognition; electrochemistry; nanostructures; thiols
Categories
Funding
- National Natural Science Foundation of China [20975075, 20771085]
- Key Program for the Basic Research of SSTCF [09JC1414100]
- Innovation Program of SECF [10ZZ21]
- State Education Ministry [NCET-06-0380]
- Nanometer Science Foundation of Shanghai [0952nm04900]
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A Pt-NiCo nanomaterial has been synthesized for developing the sensitive electrochemical determination of biological thiols that include L-cysteine (CySH), homocysteine (HCySH), and gluthione (GSH) with high sensitivity and long-term stability, in which the Pt nanoparticles are well supported on amorphous NiCo nanofilms. The electrochemical oxidation of thiols has been successfully facilitated on the optimized Pt NiCo nanostructures, that is, two oxidation peaks of CySH have been clearly observed at potentials of +0.06 and +0.45 V. The experimental results demonstrate that the first peak for CySH oxidation may be attributed to a direct oxidation from CySH to L-cystine (CySSCy), whereas the second peak possibly results from a sequential oxidation from CySSCy to cysteic acid (CySO3H), together with a direct oxidation of CySH into CySO3H. The enhanced electrocatalytic activities at the Pt-23-NiCo nanostructures have provided a methodology to determine thiols at a very low potential of 0.0 V with relatively high sensitivity (637 nA mu M cm(-2)), a low detection limit (20 nm), and a broad linear range. The striking analytical performance, together with the characteristic properties of the Pt-NiCo nanomaterial itself, including long-term stability and strong antipoisoning ability, has established a reliable and durable approach for the detection of thiols in liver cancer cells, Hep G2.
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