4.6 Article

Integrated Oligoaniline-Cross-Linked Composites of Au Nanoparticles/Glucose Oxidase Electrodes: A Generic Paradigm for Electrically Contacted Enzyme Systems

Journal

CHEMISTRY-A EUROPEAN JOURNAL
Volume 15, Issue 11, Pages 2674-2679

Publisher

WILEY-V C H VERLAG GMBH
DOI: 10.1002/chem.200801609

Keywords

biosensors; electropolymerization; glucose oxidases; gold; nanostructures

Funding

  1. MEDINGO Ltd

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Electropolymerizable glucose oxidase (GOx) was synthesized by the tethering of thioaniline units to the protein. The electropolymerization of the thioaniline-modified GOx in the presence of thioaniline-functionalized Au nanoparticles (NPs) yielded a three-dimensional bis-aniline-cross-linked Au NPs/GOx network. The bisaniline bridging units are redox active and mediate the electron transfer between the enzyme redox sites and the electrode, and the Au NPs provide the three-dimensional conductivity for transporting the electrons to the electrode. The covalently linked GOx displays an effective electrical contact with the electrode (turnover rate of ca. 500 s(-1)), which results in a sensitive and selective glucose-sensing electrode. The preparation of the electrode by means of electropolymerization, and the bioelectrocatalytic features of the electrode, suggest its feasibility as a miniaturized electrode with a sensing matrix and as an implantable glucose sensor in future applications.

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