Journal
CHEMISTRY-A EUROPEAN JOURNAL
Volume 16, Issue 3, Pages 822-828Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/chem.200902535
Keywords
glycolpepticles; glycosylation; macroporous materials; nanotechnology
Categories
Funding
- 973 Program [2010CB226901, 2007CB714506]
- NSFC [20573021, 20775016, 20925517, 20735005]
- SLADP [B108,, B109]
- STC of Shanghai [08DZ2270500, 09JC1402600]
- Shuguang [06SG02]
- Ministry of Education of China [20060246010]
- FANEDD [200423]
- Australia Research Council
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We have developed a smart nanodevice for the highly efficient and selective detection of glycoproteins. This polyfunctional device is fabricated through the rational functionalization of macroporous silica foam (MOSF) materials with a boron species (B-MOSF) and amino groups (NH2-MOSF), and then the integration of MOSF. B-MOSF and NH2-MOSF materials. In such a device, a macroporous structure with very large-pore sizes (diameters approximate to 100 nm) and high-pore volumes (>0.65 cm(3)g(-1)) is advantageous to efficiently fasten the enzymatic reaction. ne targeted specific glycopeptides of the products can be selectively isolated and enriched in B-MOSF through the chemo-affinity between boronic acid and glycol groups, while the non-specific peptides are released to the solutions, or further purified by MOSF and NH2-MOSF, which have opposite charges. As a result, the protein digestion and glycol-peptide isolation can be simultaneously achieved in the functionalized macroporous materials in one step, which is a great advantage compared to conventional multi-procedure and time-consuming techniques.
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