Journal
CHEMISTRY-A EUROPEAN JOURNAL
Volume 15, Issue 2, Pages 418-423Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/chem.200801104
Keywords
biochemical markers; dyes/pigments; fluorescence; Nile Blue; water-soluble groups
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Funding
- CEM Corporation
- National Institutes of Health [GM72041]
- Robert A. Welch Foundation
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [P20GM072041] Funding Source: NIH RePORTER
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Four water-soluble 2-hydroxy-Nile Blue derivatives, 1a, 1b, 2 a, and 2b, were prepared by condensation reactions performed under relatively mild conditions (90 degrees C, N,N-diniethylformamide with no added acid). These fluorescent probes had more favorable fluorescence characteristics than two known water-soluble Nile Blue derivatives. Specifically, they were superior to the known dyes with respect to their quantum yields in aqueous media and the sharpness of their fluorescence emissions. Concentration-dependant UV absorption and fluorescence emission studies indicated that the dyes did not aggregate in aqueous solution at concentrations of less than 1-4 mu m. The now water-soluble materials 1a, 1b, 2a, and 2b emit in a desirable region of the fluorescence spectrum (lambda=670-675nm). Overall they are potentially interesting for labeling biomolecules in aqueous environments.
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