STRUCTURE OF CARBOHYDRATE ANTIGENS FROM Microbulbifer sp KMM 6242

A capsular polysaccharide (CPS) containing D-galactosamine uronic acid and D-alanine was isolated from a culture of the marine proteobacterium Microbulbifer sp. KMM 6242. 2D NMR spectroscopy showed that the CPS is a homopolymer of 2-acetamido-2-deoxy-N-(D-galacturonyl)-D-alanine with the structure -> 4)-beta-D-GalpNAcA6(D-Ala)-(1 ->. An O-specific polysaccharide containing D-ribose and D-galactose was isolated from the cell-membrane lipopolysaccharide. 1D and 2D NMR spectroscopy established the structure of the disaccharide repeating unit of the polysaccharide as -> 3)-beta-D-Ribf-(1 -> 4)-beta-D-Galp-(1 ->.