Journal
CHEMISTRY OF MATERIALS
Volume 23, Issue 8, Pages 2113-2119Publisher
AMER CHEMICAL SOC
DOI: 10.1021/cm103557h
Keywords
conjugated polyelectrolyte; hyperbranched conjugated polymer; nanospheres; fluorescence living cell imaging; F-actin; phalloidin
Funding
- National Research Foundation [R279-000-323-281]
- National University of Singapore [R279-000-301-646]
- Ministry of Education [R279-000-255-112]
- Temasek Defence Systems Institute [R279-000-301-232]
Ask authors/readers for more resources
A phalloidin-functionalized hyperbranched conjugated polyelectrolyte (HCPE-phalloidin) is synthesized and used for direct filamentous actin (F-actin) imaging in living He la cells. Different from commercially available organic dye-phalloidin conjugates, which require sophisticated techniques to be delivered into living cells, simple incubation of living cells with HCPE-phalloidin leads to efficient internalization of the probe and clear visualization of F-actin due to high brightness of HCPE and good specificity between phalloidin and actin. In addition, HCPE-phalloidin possesses improved photostability as compared to that for commercially available Alexa Fluor 488-phalloidin conjugates, suggesting that the new probe is promising for long-term F-actin imaging in living cells. Further fine-tuning the fluorescent property and targeting ability of HCPE-based probes could lead to more complicated imaging applications and subcellular target detection.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available