Gold-Speckled Multimodal Nanoparticles for Noninvasive Bioimaging

In this report the synthesis, characterization, and financial evaluation of a multimodal nanoparticulate Contrast agent for noninvasive imaging through both magnetic resonance imaging, (MRI) and photoacoustic tomography (PAT) is presented. The nanoparticles described herein enable high resolution and highly sensitive three-dimensional diagnostic imaging through the synergistic coupling of MRI and PAT capabilities. Gadoliniurn (15d)-doped gold-speckled silica (GSS) nanoparticles. ranging from 50 to 200 Jim. have been prepared in a simple one-pot synthesis using nonionic microemulsions. The photoacoustic signal is generated from a nonuniform, discontinuous gold nanodomains speckled across the silica surface, whereas the MR contrast is provided through Gd incorporated in the silica matrix. The presence of a discontinuous speckled Surface, as opposed to a Continuous gold shell, allows sufficient bulk water exchange with the Gd ions to generate a strong MR contrast. The dual imaging capabilities of the particles have been demonstrated through in silicio and in vitro methods. The described particles also have the capacity for therapeutic