Journal
CHEMISTRY AND PHYSICS OF LIPIDS
Volume 180, Issue -, Pages 7-14Publisher
ELSEVIER IRELAND LTD
DOI: 10.1016/j.chemphyslip.2014.02.008
Keywords
Dengue; Lectin; Phospholipid; Biosensor; Glycoproteins
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Funding
- Brazilian National Council for Scientific and Technological Development (CNPq)
- Rede ELINOR de NanobiotecnologiaCAPES
- MCT/FINEP
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In this work, we developed a biosystem based on Concanavalin A (ConA) and lipid membranes to recognize glycoproteins from the serum of patients contaminated with dengue serotypes 1, 2 and 3 (DENV1, DENV2 and DENV3). The modified gold electrode was characterized using cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS) and atomic force microscopy. Morphological analyses of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC), DPPC-ConA, DPPC-ConA-DENV1, DPPC-ConA-DENV2 and DPPC-ConA-DENV3 revealed the existence of a non-uniform covering and large globules. EIS and CV measurements have shown that redox probe reactions on the modified gold electrodes were partially blocked due to the adsorption of lipid-ConA system and reveal the interaction response of the immobilized ConA to the presence of glycoproteins of dengue serum. The biosystem exhibited a wide linear response to different concentrations of sera of dengue serotypes 1, 2 and 3. A higher impedimetric response to glycoproteins present in dengue serotype 3 was observed. Our results demonstrate the applicability of lectin and lipid membranes to the development of biosensors for dengue infections. (C) 2014 Elsevier Ireland Ltd. All rights reserved.
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