Journal
CHEMISTRY AND PHYSICS OF LIPIDS
Volume 183, Issue -, Pages 184-190Publisher
ELSEVIER IRELAND LTD
DOI: 10.1016/j.chemphyslip.2014.07.002
Keywords
Drug-membrane interaction studies; Dynamic light scattering; Isoniazid; Liposomes; Small-angle X-ray scattering; Tuberculosis
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Funding
- FCT (Lisbon) [SFRH/BPD/99124/2013]
- QREN [NORTE-07-0124-FEDER-000067-NANOQUIMICA]
- [II-20100139 EC]
- Fundação para a Ciência e a Tecnologia [SFRH/BPD/99124/2013] Funding Source: FCT
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This work focuses on the application of biophysical techniques to assess the interaction of isoniazid (INH) with three-dimensional cell membrane mimetic models. Liposomes made of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) were used as cell membrane lipids, being the interactions of the drug studied under different pH conditions to mimic the in vivo near neutral physiological pH found in the cytoplasm and the acidic conditions encountered in the protective gastric barrier and in the macrophage intracellular acidic compartments (i.e., phagosomes and phagolysomes). The effect of INH on the biophysical parameters of the cell membrane lipids was assessed by dynamic light scattering (DLS) and small-angle X-ray scattering (SAXS). The overall results highlighted the importance of the pH for the interactions of INH with biological membranes. A relationship between the effect of INH on the biophysical properties of the membranes and the pH was established. In fact, the experimental results point to a higher affinity of the drug to the acidic environments, which not only explain its sequestration in the infected cells but also some of its side-effects. (C) 2014 Elsevier Ireland Ltd. All rights reserved.
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