Kinetic study of aroxyl radical-scavenging action of vitamin E in membranes of egg yolk phosphatidylcholine vesicles

Vitamin E is localized in membranes and functions as an efficient inhibitor of lipid peroxidation in biological systems. In this study, we measured the reaction rates of vitamin E (alpha-, beta-, gamma-, delta-tocopherols, TocH) and tocol with aroxyl radical (ArO center dot) as model lipid peroxyl radicals in membranes by stopped-flow spectrophotometry. Egg yolk phosphatidylcholine (EYPC) vesicles were used as a membrane model. EYPC vesicles were prepared in the aqueous methanol solution (MeOH:H2O = 7:3, v/v) that gave the lowest turbidity in samples. The second-order rate constants (k(s)) for alpha-TocH in MeOH/H2O solution with EYPC vesicles were apparently 3.45 x 10(5) M-1 s(-1), which was about 8 times higher than that (4.50 x 10(4) M-1 s(-1)) in MeOH/H2O solution without EYPC vesicles. The corrected k(s) of alpha-TocH in vesicles, which was calculated assuming that the concentration of alpha-TocH was 133 times higher in membranes of 10 mM EYPC vesicles than in the bulk MeOH/H2O solution, was 2.60 x 10(3) M-1 s(-1), which was one-seventeenth that in MeOH/H2O solution because of the lower mobility of alpha-TocH in membranes. Similar analyses were performed for other vitamin E analogues. The k(s) of vitamin E in membranes increased in the order of tocol < delta-TocH < gamma-TocH similar to beta-TocH < alpha-TocH. There was not much difference in the ratios of reaction rates in vesicles and MeOH/H2O solution among vitamin E analogues [k(s)(vesicle)/k(s) (MeOH/H2O) = 7.7, 10.0, 9.5, 7.4, and 5.1 for alpha-, beta-, gamma-, delta-TocH, and tocol, respectively], but their reported ratios in solutions of micelles and ethanol were quite different [k(s)(micelle)/k(s)(EtOH) = 100, 47, 41, 15, and 6.3 for alpha-, beta-, gamma-, delta-TocH, and tocol, respectively]. These results indicate that the reaction sites of vitamin E analogues were similar in vesicle membranes but depended on hydrophobicity in micelle membranes, which increased in the order of tocol < delta-TocH < gamma-TocH similar to beta-TocH < alpha-TocH. (c) 2011 Elsevier Ireland Ltd. All rights reserved.