Journal
CHEMISTRY & BIOLOGY
Volume 20, Issue 10, Pages 1245-1254Publisher
CELL PRESS
DOI: 10.1016/j.chembiol.2013.07.017
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Funding
- National Institutes of Health [RO1 A1073847]
- Swedish Research Council [2011-6259, 2010-4730]
- Knut and Alice Wallenberg Foundation
- Goran Gustafsson Foundation
- JC Kempe Foundation
- Umea University Young Researcher Awards
- Umea Center for Microbial Research
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Enteric bacteria assemble functional amyloid fibers, curli, on their surfaces that share structural and biochemical properties with disease-associated amyloids. Here, we test rationally designed 2-pyridone compounds for their ability to alter amyloid formation of the major curli subunit CsgA. We identified several compounds that discourage CsgA amyloid formation and several compounds that accelerate CsgA amyloid formation. The ability of inhibitor compounds to stop growing CsgA fibers was compared to the same property of the CsgA chaperone, CsgE. CsgE blocked CsgA amyloid assembly and arrested polymerization when added to actively polymerizing fibers. Additionally, CsgE and the 2-pyridone inhibitors prevented biofilm formation by Escherichia coli at the air-liquid interface of a static culture. We demonstrate that curli amyloid assembly and curli-dependent biofilm formation can be modulated not only by protein chaperones, but also by chemical chaperones.
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